Human hybridoma technology
نویسندگان
چکیده
منابع مشابه
Hybridoma Technology for Production of Monoclonal Antibodies
Hybridomas are cells that have been engineered to produce a desired antibody in large amounts. To produce monoclonal antibodies, Bcells are removed from the spleen of an animal that has been challenged with the relevant antigen. These B-cells are then fused with myeloma tumor cells that can grow indefinitely in culture (myeloma is a B-cell cancer). This fusion is performed by making the cell me...
متن کاملHuman antibodies for immunotherapy development generated via a human B cell hybridoma technology.
Current strategies for the production of therapeutic mAbs include the use of mammalian cell systems to recombinantly produce Abs derived from mice bearing human Ig transgenes, humanization of rodent Abs, or phage libraries. Generation of hybridomas secreting human mAbs has been previously reported; however, this approach has not been fully exploited for immunotherapy development. We previously ...
متن کاملProtective human hybridoma antibody to tetanus toxin.
Postimmunization human B lymphocytes and mouse myeloma cells were fused to produce interspecies hybridomas secreting human antibody of predefined specificity with an initial frequency comparable to intraspecies fusion. After 13 mo in culture, one clone continued to secrete high titers of human IgG antitetanus toxin antibody. This antibody binds to the B fragment of tetanus toxin and protects mi...
متن کاملImmunochemical techniques. Part I: Hybridoma technology and monoclonal antibodies.
Description: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today truly an essential publication for researchers in all fields ...
متن کاملAnti - Phosphocholine Hybridoma Antibodies
Three major groups of antibodies have been identified during the analysis of the clonal response to phosphoeholine (PC). t The predominant one shows multiple molecular features of the PC-binding myeloma protein (PC-BMP) TEPC 15 (1-3), and two others possess idiotypes (Id) and L-chain characteristics of two other PCBMP, M511 (2, 4) and M603 (5). All three Id groups are conserved in inbred mice (...
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ژورنال
عنوان ژورنال: Antibody Technology Journal
سال: 2012
ISSN: 2230-3170
DOI: 10.2147/anti.s30489